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human anti tra 1 85 apc  (Miltenyi Biotec)


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    Miltenyi Biotec human anti tra 1 85 apc
    Human Anti Tra 1 85 Apc, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+anti+tra+1+85+apc/TRA-1-85+(CD147)+Antibody%2C+anti-human%2C+REAfinity/pmc09974476__pnas__2205247120__sapp-45-6-10
    Average 93 stars, based on 10 article reviews
    human anti tra 1 85 apc - by Bioz Stars, 2026-10
    93/100 stars

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    Staining:

    Article Title: An HLA-G/SPAG9/STAT3 axis promotes brain metastases
    Article Snippet: BMICs were dissociated into single cells as described and resuspended in phosphate buffered saline (PBS; #311-011-CL; Wisent Inc.) containing 2 mmol/L EDTA (#15575020; Invitrogen). .. Cell suspensions were then stained with human anti-TRA-1-85-APC (1:50; 130-107-101; Miltenyi Biotec, Auburn, CA, U.S.A.), antiCD44-PE (1:50; 130-113-342; Miltenyi Biotec), anti-CD133-APC (1:50; 130-112-158; Miltenyi Biotec) or anti-HLA-G-APC (1:100; SAB4700312; Sigma-Aldrich) antibody and incubated for 15 minutes at room temperature. .. Post-incubation, the viability dye 7-Aminoactinomycin D (7AAD; 1:10; 00-6993-50; Thermo Fisher Scientific) was added to the stained cells to exclude dead cells.

    Incubation:

    Article Title: An HLA-G/SPAG9/STAT3 axis promotes brain metastases
    Article Snippet: BMICs were dissociated into single cells as described and resuspended in phosphate buffered saline (PBS; #311-011-CL; Wisent Inc.) containing 2 mmol/L EDTA (#15575020; Invitrogen). .. Cell suspensions were then stained with human anti-TRA-1-85-APC (1:50; 130-107-101; Miltenyi Biotec, Auburn, CA, U.S.A.), antiCD44-PE (1:50; 130-113-342; Miltenyi Biotec), anti-CD133-APC (1:50; 130-112-158; Miltenyi Biotec) or anti-HLA-G-APC (1:100; SAB4700312; Sigma-Aldrich) antibody and incubated for 15 minutes at room temperature. .. Post-incubation, the viability dye 7-Aminoactinomycin D (7AAD; 1:10; 00-6993-50; Thermo Fisher Scientific) was added to the stained cells to exclude dead cells.



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    Journal: Cell Reports Medicine

    Article Title: De novo GTP synthesis is a metabolic vulnerability for the interception of brain metastases

    doi: 10.1016/j.xcrm.2024.101755

    Figure Lengend Snippet:

    Article Snippet: APC-conjugated anti-human TRA-1-85 (CD147) , Miltenyi Biotec , Cat#130-128-900; RRID:AB_2921968.

    Techniques: Virus, Recombinant, Staining, Labeling, Electron Microscopy, Bradford Assay, RNA Sequencing, Gene Expression, Knock-Out, CRISPR, Luciferase, Software, Fluorescence